A new gallium complex inhibits tumor cell invasion and matrix metalloproteinase MMP-14 expression and activity
Mohsen, A.; Collery, P.; Garnotel, R.; Brassart, B.; Etique, N.; Mahmoud Shouckry, Gilane Mohamed Sabry; Elsherif Hassan, R.; Jeannesson, P.; Desmaële, D.; Morjani, H.;
Abstract
In this study, we investigated the effect of [N-(5-chloro-2-hydroxyphenyl)-l-aspartato] chlorogallate (GS2), a new water soluble gallium complex, on cell invasion and on the expression and activity of matrix metalloproteinases (MMPs) in human metastatic HT-1080 fibrosarcoma and MDA-MB 231 breast carcinoma cells. The effect on cell invasion was studied using a modified Boyden chamber coated with a type-I collagen. We analyzed the effect of GS2 on MMP-2, MMP-9, and MMP-14 via zymography and enzymatic assay using high affinity fluorogenic substrates. The expression of MMP mRNA was analyzed via qRT-PCR. GS2 induced a decrease in cell invasion. A dose-dependent inhibition effect was observed on the activities of MMP-2, MMP-9, and MMP-14 with the IC50 values of 168, 82, and 20 μM, respectively. A decrease in the expression of MMP-14 mRNA was observed in both cell lines, whereas the expression of MMP-2 and MMP-9 mRNA was decreased only in the MDA-MB231 cells. Data obtained for the expression of MMP-14 mRNA were confirmed via Western blotting. In fact, MMP-14 expression was decreased in the presence of GS2. Overall, these data show that GS2 is a promising compound for anti-invasive and anticancer therapy.
Other data
Title | A new gallium complex inhibits tumor cell invasion and matrix metalloproteinase MMP-14 expression and activity | Authors | Mohsen, A. ; Collery, P. ; Garnotel, R. ; Brassart, B. ; Etique, N. ; Mahmoud Shouckry, Gilane Mohamed Sabry ; Elsherif Hassan, R. ; Jeannesson, P. ; Desmaële, D. ; Morjani, H. | Issue Date | 2017 | Publisher | ROYAL SOC CHEMISTRY | Journal | Metallomics | DOI | 1176-1184 1756-591X 8 1184 http://www.scopus.com/inward/record.url?eid=2-s2.0-85027442334&partnerID=MN8TOARS 9 10.1039/c7mt00049a |
PubMed ID | 28765844 | Scopus ID | 2-s2.0-85027442334 | Web of science ID | WOS:000410996000016 |
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